Phage infection, transfection and transformation of Mycobacterium avium complex and Mycobacterium paratuberculosis.

Phage infection, transfection and transformation of <i>Mycobacterium avium</i> complex and <i>Mycobacterium paratuberculosis</i>

Detection of Mycobacterium avium subspecies paratuberculosis infection in two different camel species by conventional and molecular techniques

Paratuberculosis (John’s disease) is infectious and chronically progressive granulomatous disease which affects domestic and wild ruminants. The causative agent is Mycobacterium avium paratuberculosis (MAP), a slow growing mycobactin dependent acid-fast bacillus. We investigated the detection and frequency of MAP in apparently healthy dromedary and Bactrian camels by insertion...

In silico design and expression of a novel fusion protein of HBHA and high antigenic region of FAP-P of Mycobacterium avium subsp. paratuberculosis in Pichia pastoris

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy prod...

Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours.

The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a P...

Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods

Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...